Table 1. Primers for PCR-derived probes to detect restriction fragments on PFGE gels.
Fragment | Primer pairs | Length (bp) |
---|---|---|
BC, right end | 5′-GTACATGATGGATTTGACAGC-3′ | 165 |
(exon 11) | 3′-CATGCTAGCTACCCTGAGGC-5′ | |
CD, right end | 5′-CAGTCACTTGAAAATTTGATGCGAC-3′ | 153 |
(exon 29) | 3′-CTCTTCATGTAGTTCCCTCCAACGA-5′ | |
DE, right end | 5′-AATAGGAAATTGATCGGGAATTGC-3′ | 144 |
(exon 41) | 3′-GCCAGTAACAACTCACAATT-5′ | |
EF, left end | 5′-GAATATAAAAGATAGTCTACAACA-3′ | 175 |
(exon 43) | 3′-CCCTTGTCGGTCCTTGTAC-5′ | |
FG, right end | 5′-TTCAGAATCAGTGGGATGAA-3′ | 165 |
(exon 54) | 3′-TATAGGGACCCTCCTTCCAT-5′ |