Table 2. Primers for PCR-derived probes for in situ hybridization.
Probe for | Primer pairs | Length (bp) |
---|---|---|
Loop 1 | 5′-GCCTAAAAAGTGTTTCAATAGAGG-3′ | 2019 |
3′-AAAGTCGACCTGATGTGAAATCC-5′ | ||
AR 3 | 5′-AATAATTGGACATTTCTCTCGCTAG-3′ | 2334 |
3′-ATTTAGAGAGGGAAAGCCTTTCAA-5′ | ||
Loop 3 | 5′-GGCAGTGCCCTTTGGGTATTTCT-3′ | 2749 |
3′-CGAGCCTGCCATGAGTTTAAAAT-5′ | ||
AR 4 | 5′-ATTAATCCTAATGCTACAGCTTCAG-3′ | 4125 |
3′-AAATGATAGCCCCTGCATAGACAG-5′ | ||
AR 5 | 5′-TGTGTTTGAATGAAGAGAAAGGCA-3′ | 5012 |
3′-GGACCATTAATGGGACTTAGAAAT-5′ | ||
AR 7 | 5′-ACTATAATACCATATAGGCAATGCC-3′ | 2118 |
3′-GATAAATGAGGGGAATGCTGATTT-5′ | ||
Loop 7 | 5′-AAAGATGAGCTTTTCCATGCTAGT-3′ | 3858 |
3′-AGTAGAAAGGTTTATCCCAGGCCA-5′ | ||
AR 8 | 5′-ATTTGTTAAAAGTTAGCCCCTCCA-3′ | 3779 |
3′-TTACCAAATCTGTTCCTCTTCCTA-5′ |
AR, attachment region.