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Effect of mutations in HTR2C minigenes on RNA splicing. RT–PCR products from RNA in SH-SY5Y cells transfected with minigenes. Wt, wild-type; m1-2, Δ1-2, as indicated in Figure 1; letters alone, editing site with A > G mutation; subscript T, A > T; subscript C, A > C; asterisk, compensatory mutation. The positions of PCR products corresponding to RNAGU1, RNAGU2, RNAGU3 and RNAGU1/RNAGU2 hybrid are as indicated.
