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. Author manuscript; available in PMC: 2014 Jul 2.
Published in final edited form as: Cancer Res. 2012 Sep 24;72(22):5856–5866. doi: 10.1158/0008-5472.CAN-12-1635

Figure 5. PI3K/mTOR inhibition fully blocks Ras phenotypes in basal but not luminal MECs.

Figure 5

BasalRAS/GFP and luminalRAS/GFP organoids were cultured under the indicated treatment conditions for 6 d. Left panels depict day 6 images (10X) of representative organoids for each genotype and treatment condition (see also Videos 7–10). Morphometry of 2D images captured at days 0, 2, 4 and 6 were used to determine the prevalence of focal invasion, as well as the mean organoid circularity and area at each time point. (A) PI3K pathway inhibition. LY denotes PI3K inhibitor LY294002 at 10 μM. Each data point reflects the analysis of 24 to 47 organoids. (B) mTOR pathway inhibition. Rap denotes rapamycin at 5nM. Each data point reflects the analysis of 18 to 114 organoids. Error bars denote SEM.