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. 2004 Apr 23;32(7):2281–2285. doi: 10.1093/nar/gkh543

Figure 1.

Figure 1

Cleavage of target RNA by DzF and LzF4. Each was incubated with a 32P-labelled 388 nt human EGR-1 transcript for 3 h at 37°C at the indicated stoichiometric ratios. Upon termination of the reaction, cleavage products were denatured and resolved by electrophoresis on 12% polyacrylamide denaturing gels. DzFSCR and LzF4SCR were used in parallel at the indicated stoichiometries.