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DNAzyme blockade of serum-inducible EGR-1 protein expression. Subconfluent growth-quiescent SMCs were transfected with 0.2 µM of either DzF or LzF4 in 100 mm tissue culture dishes. Protein lysates were harvested from cells 1 h following a second transfection in the presence of 5% FBS. Lysates were assessed for EGR-1 and YY1 immunoreactivity by western blot analysis using polyclonal antibodies to EGR-1 and YY1. ODN denotes oligonucleotide.
