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Primer length requirement of the telomerase-mediated cleavage activity at different primer concentrations. DNA primer extension assays were performed with the indicated oligonucleotides. The elongation products were resolved on 10% polyacrylamide-urea gels. The 3′-end radiolabeled 5′-biotinylated primers, (T2AG3)3 and (T2AG3)4, migrate, respectively, as 19 and 25mers at the positions indicated by the arrows. Numbers represent the lengths of the elongation products.
