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. 2014 Jul 2;4:5531. doi: 10.1038/srep05531

Figure 2. The expression of thrombin was increased in the ischemic regions of MCAO rats.

Figure 2

(A–B) The immunoreactivity of thrombin in the cortex and striatum of ischemic brain tissue. Scale bar = 100 μm. Significant thrombin antigen was found in regions of the ipsilateral hemisphere striatum. (C) Double staining examining the colocalization of thrombin with FITC-dextran staining in the striatum and cortex. Scale bar = 50 μm. (D) Significant FITC-dextran leakage present in the ipsilateral hemisphere striatum. (E) Significant amounts of thrombin were expressed in areas with severe FITC-dextran leakage regions. (F) Double staining examining the colocalization of thrombin with MAP-2-positive neurons. Scale bar = 50 μm. Immunohistochemistry with an anti-thrombin antibody and a neuronal marker anti-MAP-2 antibody revealed thrombin expression mostly in ischemic neurons. The white arrow indicates the colocalization of thrombin staining with FITC-dextran or neurons. ***P < 0.001, n = 6–8.