Figure 2. Phylogenetic analysis of selected human, fly and mosquito biogenic amine receptors.

Protein sequences of each receptor (“R”) were first aligned in Muscle⁴⁷ and then a Maximum-Likelihood tree was constructed in MEGA 6 using 1000-fold bootstrap re-sampling. All insect receptors are shown in green, while the human receptors are highlighted in blue. The numbers at the nodes of the branches represent the level of bootstrap support for each branch. The D.melanogaster FMRF amide receptor (DmFR, AAF47700.1) was used as outgroup. The accession numbers for each receptor are listed in Supplementary Table S1.