Figure 3. Markers of lipid metabolism in liver.

Triacylglycerol content (A) was used as a measure of lipid accumulation. The activity of medium chain acyl coenzyme A dehydrogenase (MCAD, B), citrate synthase (CS, C) and acyl-CoA oxidase (AOX, D) were determined in liver homogenates as described previously¹⁶. Protein levels of oxidative and peroxisomal markers in liver were measured by immunoblotting (E–H) as described in⁸,⁵³. Oxidation of [1-¹⁴C]-palmitic acid to CO₂ (I) and acid-soluble metabolites (ASM, J) was assessed in liver homogenates. Shown are means ± SEMs; with n = 8–10 mice per group. Representative immunoblots show n = 2, with densitometry calculated for n = 8–10 per group. a,b,c p < 0.05, 0.01, 0.001 vs chow; d, f p < 0.05, 0.001 vs lard-HFD; * p < 0.05, † p < 0.01, ‡ p < 0.001. Complex I, III and V represent sub-units of the complexes of the electron transport chain (ETC), PMP70 = peroxisomal membrane protein 70. AU – Arbitrary units.