Figure 5. Markers of lipid metabolism in skeletal muscle.
Triacylglycerol content (A) in quadriceps muscle was used as a measure of lipid accumulation. Beta-hydroxyacyl dehydrogenase (β-HAD; B), medium chain acyl coenzyme A dehydrogenase (MCAD, C) citrate synthase (CS, D) and acyl-CoA oxidase (AOX, E) activities were measured in muscle homogenates. Oxidation of [1-14C]-palmitic acid was assessed in muscle homogenates, with the ratio of CO2:acid soluble metabolites (ASM) shown (F). Shown are means ± SEMs; with n = 8–10 mice per group. a,b,c p < 0.05, 0.01, 0.001 vs chow; f, p < 00.001 vs lard; * p < 0.05, ‡ p < 0.001.