Figure 4.

Real-time PCR quantification using displacing probes. PCR was conducted using the primer pair and the wild-type probe for IVS-2–654. Ten-fold serial dilutions of each genomic DNA template, ranging from 7.5 × 10²–7.5 × 10^–3 ng (A, from left to right) per 25-µl reaction and the negative control were amplified. The linear relationship (B) between the number of threshold cycle values and the logarithm of the mass amount of genomic DNA present in a sample demonstrated that quantitative determinations could be made over a target concentration range of at least six orders of magnitude.