Figure 6.

Simultaneous real-time PCR genotyping of HBB gene containing five mutations. Fluorescence was recorded at both Fam and Texas Red channels corresponding to the wild-type and mutant probes, respectively. For each mutation, typical three PCR results were shown corresponding to the three genotypes of the templates, they were wild-types (black solid lines), mutant types (dashed lines) and heterozygotes (dash-dotted lines). Templates used were artificial plasmid DNA. Negative control is shown as a gray solid line.