Figure 2.

Flow cytometry histograms of apoptosis assays by PI method in N2A cells. Flow cytometry histograms of PI-stained N2A cells treated with different concentration of safranal for 48 h. Sub-G1 peak as an indicative of apoptotic cells, was induced in safranal-treated but not in control cells. Flow cytometry histogram of positive control in which cells were cultured in serum free medium and safranal-treated cells. Safranal -treated cells exhibited a sub-G1 peak in N2Aells that indicates the involvement of an apoptotic process in safranal-induced cell death