Table 1. Thermal duplex stabilities of different LNA-substituted oligo(T) affinity probes.
| ID | Sequence (5′→3′)a | Tm values (°C)b ribo-A20 complement |
|---|---|---|
| LNA_T20 | Biotin-TTTTTTTTTTTTTTTTTTTT | >95 |
| LNA_T15 | Biotin-TTTTTTTTTTTTTTT | >95 |
| LNA_T10 | Biotin-TTTTTTTTTT | 76.3 |
| LNA_2.T | Biotin-TtTtTtTtTtTtTtTtTtTt | 70.8 |
| LNA_3.T | Biotin-TttTttTttTttTttTttTt | 60.8 |
| LNA_4.T | Biotin-ttTtttTtttTtttTtttTt | 56.9 |
| DNA-oligo(dT)20 | Biotin-tttttttttttttttttttt | 40.3 |
aT (upper case), 2′-O,4′-C-methylene-d-ribofuranosyl (LNA) thymidine; t (lower case), thymidine; Biotin, biotin–(CH2)4–CONH–(CH2)6–oligonucleotide sequence.
bMelting temperature (Tm values) were obtained from the maxima of the first derivatives of the melting curves (A260 versus temperature, 15–95°C with a 1°C increase per min) recorded in Tm buffer using 1.0 µM concentrations of the two complementary strands.