Table 1. Sequences of primers, and DNA and PNA hybridization probes.
Sequence | |
---|---|
Primers | |
F1 | 5′-GAGCCTGACCTTGTAGAGAGAC-3′ |
R1 | 5′-GGGTTCTTCTCTATAAACAGTGC-3′ |
R2 | 5′-GGTGAATGTCAAGAAACCATGA-3′ |
Hybridization probes | |
FDP R201 DNA | 5′-CCGTGTCCTGACTTCTGGAATC-3′ |
FDP R201H DNA | 5′-CCATGTCCTGACTTCTGGAATC-3′ |
FDP R201C DNA | 5′-CTGTGTCCTGACTTCTGGAATC-3′ |
RAP705 DNA | 5′-TTGAGACCAAGTTCCAGGTGGACAAA-3′ |
FDP DNA | 5′-GGTGAGATCCATTGACCTCAATTTTGTTTCAGGACCTGCT-3′ |
RAP640 R201 PNA | 5′-CGCTGCCGTGTCCT-3′ |
RAP640 R201H PNA | 5′-CGCTGCCATGTCCT-3′ |
RAP640 R201C PNA | 5′-CGCTGCTGTGTCCT-3′ |
The underlined letters represent single nucleotide mutations. F1 = forward primer, R1 = reverse primer, R2 = reverse, more 5′ primer, FDP = fluorescein donor probe, RAP = Red acceptor probe.