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. 2004 Apr 19;32(7):e63. doi: 10.1093/nar/gnh059

Table 1. Sequences of primers, and DNA and PNA hybridization probes.

  Sequence
Primers  
 F1 5′-GAGCCTGACCTTGTAGAGAGAC-3′
 R1 5′-GGGTTCTTCTCTATAAACAGTGC-3′
 R2 5′-GGTGAATGTCAAGAAACCATGA-3′
Hybridization probes  
 FDP R201 DNA 5′-CCGTGTCCTGACTTCTGGAATC-3′
 FDP R201H DNA 5′-CCATGTCCTGACTTCTGGAATC-3′
 FDP R201C DNA 5′-CTGTGTCCTGACTTCTGGAATC-3′
 RAP705 DNA 5′-TTGAGACCAAGTTCCAGGTGGACAAA-3′
 FDP DNA 5′-GGTGAGATCCATTGACCTCAATTTTGTTTCAGGACCTGCT-3′
 RAP640 R201 PNA 5′-CGCTGCCGTGTCCT-3′
 RAP640 R201H PNA 5′-CGCTGCCATGTCCT-3′
 RAP640 R201C PNA 5′-CGCTGCTGTGTCCT-3′

The underlined letters represent single nucleotide mutations. F1 = forward primer, R1 = reverse primer, R2 = reverse, more 5′ primer, FDP = fluorescein donor probe, RAP = Red acceptor probe.