Figure 3.

Position of the antisense oligonucleotides used in this study. (A) The selected target sites superimposed on the BMH secondary structure of the HIV-1 HXB2 subtype leader drawn according to Damgaard et al. (21). Thick lines denote the sequences complementary to the selected RNA oligonucleotides after five rounds of selection. The thin line denotes the sequence complementary to the PSI-specific oligonucleotides that was obtained frequently after the third round of selection, but only once after the fifth round (Table 1). The major structure elements are denoted (see text for details). (B) The annealing sites for DIS_upstream–, DIS_skripkin– and the selected DIS–oligonucleotides indicated by dotted, stippled and full lines respectively. (C) The annealing sites for PBS_nat– and PBS–oligonucleotides.