Figure 4.

HIV-1 leader RNA dimerization assay. HIV-1 leader RNA (+1–355) was incubated under dimerization conditions in the absence (Control), in the presence of oligonucleotide CN1 (lane 2), DIS_skripkin–DNA (lane 3) or selected antisense DNA and RNA oligonucleotides as denoted above (see Table 2 for sequences). The dimerization efficiency, indicated below, was calculated as [dimer]/[dimer]+[monomer] from three independent experiments. The positions of dimeric (D) and monomeric (M) RNA species are indicated.