Figure 5.

Pausing of reverse transcription by selected oligonucleotides. Binding of the selected oligonucleotides (Table 2) and their ability to block reverse transcription was investigated by a reverse transcription progressivity assay using an extended HIV-1 template (+1–444). A 5′-end-labelled DNA primer complementary to position 384–401 of the viral RNA was used to prime reverse transcription. A control without oligonucleotide (lane 1) and a sequencing ladder (lanes 7–10) are included.