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Time course for the disruption of preformed HIV-1 dimer into RNA monomers in the presence of DIS-specific oligonucleotides. One picomole of radiolabelled HIV-1 leader RNA was dimerized and challenged with 5 pmol DIS–RNA or DIS–DNA for 0, 30, 120 or 240 min at 37°C before native gel electrophoresis. The dimerization efficiency was calculated as [dimer]/[dimer]+[monomer] from three independent experiments.
