Table 1.
Drug VP 16 (Dose) | Mean % survival with VP 16(n = 5) | Drug with PC3 cells | Endlabelling Fluorescence (A.U.) | ||
LNCaP | PC3 | DU145 | Control Taxotere | 2 | |
2 μg/ml | 85 | 96 | 75 | 10 nM | 10 |
5 μg/ml | 80 | 84 | 70 | 50 nM | 10 |
10 μg/ml | 85 | 82 | 65 | Campothecin | |
20 μg/ml | 80 | 72 | 55 | 200 nM | 135 |
Notice the most pronounced response of DU 145 followed by PC3 and LNCaP cell lines against VP 16 (20 μg/ml). Dynamic range of the mean endlabelling fluorescence intensity was normalized to arbitrary control value of 1.0 non-linear scale (n = 5). The combination of taxotere on PC3 was apparently saturated at 10 nM for 24 hours at about 10% of the campothecin dose, a near maximal response.