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Effect of HdCDT on HaCat keratinocytes. Growing cells were exposed to 122 ng/ml HdCDT for 2 h at 37°C. After postincubation in medium for 48 h (a) or 24 h (b), cells were fixed and stained with Giemsa. (c) Control cells, 48 h. ×100. HdCDT, Haemophilus ducreyi cytolethal distending toxin.