Figure 6.

DNA synthesis in HdCDT-treated cells. (a) Incorporation of BrdU. HEp-2 cells treated with HdCDT for 15 min on ice were postincubated for 18, 24, and 36 h in medium at 37°C. BrdU (10 μg) was present during the final 12 h, and its incorporation was visualized by staining with anti-BrdU. (b) Incorporation of [³H]thymidine. HEp-2 cells treated with HdCDT as described above were postincubated for 6, 12, and 24 h. [³H]thymidine (1 μCi/ml) was present during the final 30 min, and its incorporation in DNA was determined as detailed in Methods. Mean values + SD of three different experiments. BrdU, bromodeoxyuridine.