Figure 1.

Age-dependent increase in the latency to flurothyl-induced seizure in mnd mice. Male wild-type, mnd, and nclf mice at 3 different ages (21 days to 25 days, 35 days to 42 days, and 6 months to 9 months) were used in the experiments. Seizure was induced by inhalation of flurothyl gas, and the latency until loss of posture was measured. Columns and bars represent mean ± standard error of the mean (n = 4–7). At the age of 35 days to 42 days, the mean latency to flurothyl-induced loss of posture was 532.1 ± 47.8 s for mnd mice and 420.9 ± 17.6 s for 35-day to 42-day wild-type mice (P < .05 by unpaired t test). No significant differences were observed between mnd and wild-type mice at earlier (21 days to 25 days) or later (6 months to 9 months) developmental time points. No statistically significant differences existed between nclf and age-matched wild-type mice.