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. 1999 Jan 1;103(1):27–37. doi: 10.1172/JCI4431

Figure 2.

Figure 2

Genotyping of the Ins2 gene by RFLP analysis. Ins2 exon 3 was amplified using PCR from genomic DNA. The left lane shows ϕX174/Hae III-digested DNA markers. (a) The size of PCR products derived from C57BL/6J (C1, C2, and C3) or Mody mice (A1, A2, and A3) was 280 bp. The mutation found in Mody mice, described in Fig. 1, disrupts an Fnu 4HI site in the exon 3 of Ins2. Digestion with Fnu 4HI did not change the size of the PCR products from the mutated allele (280 bp) but decreased that of the wild-type allele to 140 bp. (b) Representative genotyping of 16 offspring derived from three Mody congenic lines with C3H/He background is shown. Mice with diabetes are shown as “+” under the lane number. The genotype of Ins2 was completely matched with the phenotype in each individual.