Figure 2.

Immunoassay showing binding of MABs EO3, EO6, EO7, EO14, and MDA2 to the protein moiety (upper row) or to the lipid moiety (lower row) of native LDL (squares), LDL oxidized with copper for 4 h (circles), and LDL oxidized with copper for 18 h (triangles). Protein and lipid moieties of native and OxLDL preparations were separated with chloroform/methanol extraction, and the extracted lipids were made into microemulsions by extrusion. Then, 10 μg/ml of the MABs was incubated with antigens, and the amount of antibody bound was measured with alkaline phosphatase–labeled goat anti–mouse IgM (or IgG) secondary antibody. Each point is the mean of triplicate determinations.