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Binding of IgG-CD59 fusion proteins containing conformation-sensitive CD59 epitopes to dansyl. Purified IgG-CD59 fusion proteins (100 ng/ml) or anti-dansyl IgG4 control antibody were incubated in dansylated BSA–coated microtiter plates. Using standard ELISA technique, bound IgG-CD59 was determined using a panel of anti-CD59 MABs that recognize conformational epitopes on CD59. DNS, dansyl; MABs, monoclonal antibodies.
