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McNtcp.24 cells express Fas receptor but not FasL mRNA. After McNtcp.24 cells were cultured for 2 h in the presence or absence of 50 μM GCDC, total RNA was isolated for subsequent RT-PCR using Fas, FasL, and GAPDH. RNA from ileum served as a positive control for both Fas receptor and FasL. The identities of the PCR products were verified by DNA sequencing. FasL, Fas ligand; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT, reverse transcriptase.
