Figure 8.

GCDC treatment of primary mouse hepatocytes cells results in Fas receptor oligomerization. Untreated and GCDC (50 μM)-treated cells were incubated with the cleavable cross-linking reagent 3,3′-dithio-bis(sulfosuccinimidyl proprionate) and lysed for immunoprecipitation using limiting (0.5 μg/ml) or excess (5 μg/ml) amounts of anti-Fas antibody. Western blot analysis of the immunoprecipitate was performed as described in Methods.