Figure 5. Chemoradiation and intratumoral dendritic cell administration increase Th1 cytokines IFN-γ and IL-2 in both CD4+ and CD8+ T cells after stimulation with SCCVII tumor.
Spleens from naïve mice and mice with 29-day tumor after completion of the various treatment regimens were harvested. Splenocytes were cultured for 24 hours with either media alone or with SCCVII tumor cells (5:1). Intracellular cytokine staining for IFN-γ (Figure 5A) and IL-2 (Figure 5B) and analysis by flow cytometry were performed. (*) p < 0.05 compared to media-cultured (control) cells.