Figure 5.

Collagen induces tyrosine phosphorylation of Syk but not PLC-γ2 in SLP-76–deficient platelets. Platelets were isolated from SLP-76 +/+, +/–, or –/– mice and left resting (basal, B) or stimulated with collagen (C) for 90 s. Platelets were then lysed and subjected to immunoprecipitation with Syk-specific (top panels) or PLC-γ2–specific (bottom panels) antibodies. Immunoprecipitates were washed, resolved by SDS-PAGE, and then transferred to nitrocellulose. Filters were then immunoblotted with phosphotyrosine-specific antibody (α-pTyr). Filters were then stripped and reblotted with Syk- or PLC-γ2–specific antibodies to demonstrate equivalent sample loading.