Figure 2. TNFα-induced ERK3 expression is not regulated by NF-κB pathway.
HUVECs were transiently transfected with p65 siRNA (p65si), p50 siRNA (p50si), or the silencer negative control siRNA (Ctrlsi), then serum-starved overnight, followed by the treatment with TNFα or vehicle. Gene expression was determined by RT-qPCR. ns: no significance.
