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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Cell Physiol. 2014 Oct;229(10):1529–1537. doi: 10.1002/jcp.24596

Figure 3. ERK3 expression is regulated by TNFα through c-Jun.

Figure 3

HUVECs were transiently transfected with c-Jun siRNA (c-Junsi), c-Fos siRNA (c-Fossi), or the silencer negative control siRNA (Ctrlsi). Cells were then treated with TNFα (10 ng/ml) or vehicle for 6 hours. Knockdown of c-Jun (A) and c-Fos (B) was verified by RT-qPCR. ERK3 gene expression was determined by both RT-qPCR (Bar-graph) at mRNA level and by Western blotting at protein level (C). ERK3 IB: ERK3 immunoblot.