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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Cell Physiol. 2014 Oct;229(10):1529–1537. doi: 10.1002/jcp.24596

Figure 7. Knockdown of ERK3 downregulates VEGFR2 expression and signaling.

Figure 7

HUVEC cells were transiently transfected with ERK3 siRNA (ERK3si) or the silencer negative control siRNA (Ctrlsi). (A). Verification of ERK3 knockdown by RT-qPCR. (B). RT-qPCR analysis of VEGFA mRNA expression. ns: no significance. (C). RT-qPCR analysis of VEGFR2 mRNA expression. (D). Western blot analysis of VEGFR2 protein expression. Actin was probed as a loading control. (E). HUVEC cells were transfected with ERK3 siRNA (ERK3si) or the silencer negative control siRNA (Ctrlsi), starved in serum-free medium, and then treated with or without VEGFA (25 ng/ml) for 10 min. Protein expression of VEGFR and ERK1/2 and phosphorylation of ERK1/2 (P- ERK1/2) were analyzed by Western blotting.