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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Cell Physiol. 2014 Oct;229(10):1529–1537. doi: 10.1002/jcp.24596

Figure 8. ERK3 and SRC-3 synergistically regulate SP1-mediated VEGFR2 gene promoter activity.

Figure 8

(A). HUVEC cells were transfected with SRC-3 siRNA (SRC-3si) or the non-targeting negative control siRNA (Ctrlsi). Expression levels of SRC-3 and VEGFR2 were analyzed by Western blotting. (B). A schematic illustration of a VEGFR2 gene promoter-driven luciferase reporter construct (pGL3-VEGFR2-Luc). A SP1 binding element resides in the VEGFR2 gene promoter. (C) and (D). ERK3, SRC-3 and SP1 synergistically promote VEGFR2 promoter-driven luciferase activity. HUVECs were transfected with pGL3-VEGFR2-Luc and SRC-3, ERK3, or SP1 construct as indicated. Luciferase activity is represented as relative luciferase units (RLU). (E). Knockdown of SRC-3 diminished the stimulatory effect of ERK3 and SP1 on VEGFR2 gene promoter-driven luciferase activity. HUVECs were transfected with pGL3-VEGFR2-Luc and ERK3 or SP1 construct, together with either SRC-3 siRNA (SRC-3si) or non-targeting control siRNA (Ctrlsi) as indicated.