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. 1999 Jan 15;103(2):185–195. doi: 10.1172/JCI3326

Figure 4.

Figure 4

Effect of a PKC-β–specific inhibitor, LY333351, on p38 MAP kinase activity in rat aortic SMC. After 3 days of exposure to several concentrations of glucose or mannitol, the cells were untreated or treated with a PKC-specific inhibitor, LY333351 [(a) 20 nM and (b) 500 nM], for 30 min, and then lysed. The p38 MAP kinase activity was quantitated by the phosphorylation of MBP using [γ-32P]ATP as described in Methods. LY333351 was solubilized with DMSO. The results were derived from four separate experiments, with each experiment performed in duplicate. Each bar represents the mean ± SEM. *P < 0.05 vs. 5.5 mM glucose.