Figure 6.

Effect of PKC-β1 overexpression on p38 MAP kinase in rat aortic SMC. (a) Characterization of PKC-β1 overexpression in rat aortic SMC after adenovirus infection. (1) Total cell lysate was isolated from cells that were not infected (cont.), or infected with Adv-CMV-βGal (β-gal) or Adv-CMV-PKC-β1 (PKC-β1). PKC-β1 protein was detected by immunoblot analysis. (2) Four days after infection with adenovirus, cells were lysed and the cytosol and membrane proteins were fractionated by ultracentrifugation. The PKC activity was measured as described in Methods. Each bar represents the mean + SD (n = 5). *P < 0.001 vs. noninfected and β-gal. (b) Activation of p38 MAP kinase in PKC-β1–overexpressed SMC. Four days after infection with adenovirus, total cell lysate was isolated. The samples were separated by 10% SDS-gel, and the phosphorylation of p38 MAP kinase was detected by immunoblot analysis using phosphospecific p38 MAP kinase as described in Methods. The results were derived from three separate experiments.