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. 2013 Jun 1;73(7):1405–1413. doi: 10.1136/annrheumdis-2012-203146

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Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 3.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/3.0/

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The p53/p21 pathway is activated in vivo following local injection with glucocorticoids. Tendon biopsy samples were taken from five patients with rotator cuff tendinopathy immediately prior to subacromial injection with the synthetic glucocorticoid, Depo-Medrone, and compared with subsequent biopsy specimens taken from the same patients 7 weeks following glucocorticoid injection. Using immunohistochemistry, (A) the percentage of p53-positive cells was significantly greater in tissue samples post-injection compared with pre-injection. (B) A non-statistically significant increase in the percentage of p21-positive cells was observed in the post-injection tissue samples compared with pre-injection tissue samples. Representative photomicrographs are shown for tissue samples from two patients. p53-positive cells or p21-positive cells were detected using horseradish peroxidase-conjugated secondary antibodies and diaminobenzadine. Positive cells appear brown. Tissue was counterstained with haematoxylin (blue) to allow quantification of total cell numbers. Statistically significant differences are indicated by * (p<0.05).