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. 1999 Jan 15;103(2):197–206. doi: 10.1172/JCI3523

Figure 8.

Figure 8

(a) Effect of TGF-β on PTHrP secretion by MDA-MB-231 and MDA/TβRIIΔcyt cell clones that overexpress PTHrP (TβRIIΔcyt + PTHrP; two clones) or the empty vector (TβRIIΔcyt + pcDNA3.1zeo). Respective cells were plated onto 48-well plates and treated as in Fig. 1a. Values represent the mean ± SEM (n = 3 per group). (b) Representative radiographs of hindlimbs from mice bearing two different TβRIIΔcyt + PTHrP clones or TβRIIΔcyt + pcDNA3.1zeo control 31 days after tumor inoculation. Osteolytic lesions are indicated by the arrows. (c) Osteolytic lesion number and area on radiographs as measured by computerized image analysis of forelimbs and hindlimbs. Respective tumor cells were inoculated on day 0. Values represent the mean ± SEM (n = 5) per group. (d) Plasma PTHrP concentrations at sacrifice were significantly higher than respective concentrations prior to tumor inoculation (baseline) in mice bearing either TβRIIΔcyt + PTHrP tumors. There was no significant difference between baseline and sacrifice values in mice bearing the control TβRIIΔcyt + pcDNA3.1zeo tumors.