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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: J Immunol. 2014 May 14;192(12):6111–6119. doi: 10.4049/jimmunol.1302895

Figure 7. Depletion of MDSCs improves survival to endotoxemia in 4T1 bearing animals.

Figure 7

(A) Balb/C J 28 days post 4T1 inoculation were injected with either PBS or clodronate liposomes (Encapsula nanosciences, Nashville TN) IV at 100 uL of Liposome suspension/ 10 g of body weight. Animals were then euthanized after 48 hours. (B) Splenocytes from either clodronate (n=5) or PBS (n=3) injected animals were stained for CD11b+Gr-1+ cells and absolute numbers of MDSCs were then calculated (* Student t-test p<0.001). (C) 4T1 and NT-bearing mice at 28–30 days post tumor inoculation were administered either Clodronate or PBS liposomes IV. After 36–48 hours mice were then injected with LPS (50 µg) ip and followed for 72 hours (* Fisher’s exact test NT vs 4T1 +PBS p<0.001, ** 4T1 + Clodronate vs 4T1 +LPS p =0.013). Panel represents the summary of two experiments.