Fig. 1.
Nherf1 in the mouse auditory hair cells. (A) Nherf1 immunostaining is detected on embryonic day 16 in the stereocilia of IHCs and OHCs. After birth (P0 and P12), Nherf1 was detected only in OHC hair bundles. Nherf1 labeling was intense at the tips of the differentiating stereocilia. (B and C) Nherf1 and cadherin-23 colocalized at the stereocilia tips. On P5, Nherf1 labeling is weaker in the stereocilia of hair cells, but not in the supporting cells (arrows in B and C), in Cdh23v2j/v2j (C) than in control (B) mice. (Scale bars, 5 µm.)