Fig. 4.

Mutations that prevent ethanol from accessing K361 abolish ethanol-induced increase in mslo1 channel activity. (A) Ethanol responses of mslo1-E354Y (n = 5), mslo1-S357Y (n = 3), and mslo1-N358W NPo (n = 4) vs. WT mslo1; **P < 0.01. (B) At $\mathrm{C}{\mathrm{a}}_{\mathrm{i}}^{2+}$= 0.3 mM, WT mslo1, mslo1-E354Y, mslo1-S357Y, and mslo1-N358W have similar V_half. (C) Molecular surface map highlights the location of N372, E374 and S382 outside the ethanol site. (D) Ethanol-induced increases in NPo are similar in mslo1 (n = 10), mslo1-N372W (n = 5), mslo1-E374Y (n = 5), and mslo1-S382Y (n = 4).