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Role of the ethanol site in long-chain alkanol effects on mslo1. Heptanol increases WT mslo1 (A) but not mslo1-K361N NPo (B). (C) Averaged responses to heptanol from mslo1 (n = 4) vs. mslo1-K361N NPo (n = 3). (D) Poses of n-alkanols on the ethanol site surface map. Ethanol C backbone is in pink; other activatory n-alkanols are shown in yellow, whereas ineffective n-alkanols are in orange. In all cases, oxygen is in red. Hydrogen bond is a dotted line.
