Fig. 4.

Reversions of the F8 gene inversion. (A) PCR analysis is of genomic DNA from three reverted clones. Genomic DNAs isolated from hemophilia A patient cells (Pa) or wild-type iPSCs (WT) served as positive controls for inversion or normal genotypes, respectively. (B) DNA sequences of breakpoint junctions in reverted clones. TALEN binding sites are shown in red (junction 1) or blue (junction 2). Dashes indicate deleted bases. The chromatograms show the sequences (of homolog 1 and 2, respectively) between two TALEN binding sites in reverted clones (clones 1 and 3).