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. 2014 Jun 9;111(25):9151–9156. doi: 10.1073/pnas.1323265111

Fig. 2.

Fig. 2.

Assembly of primary cilia regulates proliferation of myoblasts. (A–D) qRT-PCR detection of knockdown efficiency in C2C12 myoblasts using miRNAs. Two different miRNAs targeting Cep290 (A), IFT80 (B), and IFT88 (C) were analyzed. Empty vector and a nonspecific (NS) miRNA were used as controls. Relative expression values are shown; error bars, SEM. (D) Quantification of cilia percentages in C2C12 cells expressing different miRNAs. (E) Quantification of percentages of Ki-67-positive cells. (F) Quantification of percentages of EdU-positive cells. Data are shown as mean ± SD. In each case, **P < 0.01.