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DNase I protection assay with RNAP in the presence or absence of Ler on the LEE1 P1 noncoding strand as determined by primer extension.[γ-32P] ATP-labeled PCR products (−134 to +87) were treated with DNase I in the presence of 20 nM RNAP or 60 nM Ler or Ler plus RNAP, or no protein as indicated above each lane. Sequencing marker lane is indicated. The density plot on the right shows the pattern in the presence of no protein (red), Ler (green), RNAP (blue), and Ler plus RNAP (magenta).
