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. 1999 Jan 15;103(2):271–279. doi: 10.1172/JCI5205

Figure 5.

Figure 5

Effect of transgene expression on ligand-independent activation of TSH. (a) TSH concentrations were measured 3–4 weeks after the mice were rendered hypothyroid by iodine-131 administration. TSH levels rose markedly in TG (40-fold) and WT (>400-fold) when compared with their respective baseline values. However, the rise in TSH was 10-fold greater in WT mice. Number of animals in each group are WT: n = 13; line 1: n = 9; and line 2: n = 5. **P < 0.01, *P < 0.05 vs. WT. (b) TSH-β mRNA in WT and TG hypothyroid mice (pooled pituitaries). A Northern blot was performed using serial dilutions of pituitary total RNA. Data from line 1 are shown. Similar data (not shown) exist for line 2 mice. The blots were initially hybridized with the mouse TSH-β cDNA, and then with actin. Densitometry determined mRNA abundance, and actin expression was used to adjust the TSH-β message for differences in loading. TSH-β mRNA abundance was 40% lower in TG vs. WT mice.