Fig. 4.

The C terminus of perilipin 1 is required for the binding and stabilization of ABHD5. A schematic illustration of the domains of perilipin 1 that were suggested to be homologous to perilipin 3 by Hickenbottom et al. (33) is provided for orientation. Numbering throughout this figure refers to the amino acid numbering of human perilipin 1. COS-7 cells were cotransfected with Yn-ABHD5 along with Myc-perilipin 1 WT and/or Myc-perilipin 1 mutants and detected by immunoblotting (A–E). Blots are representative of two independent experiments. In parallel, competitive BiFC was performed to assess the ability of the perilipin mutants to interfere with interaction between ATGL (S47A) and ABHD5 (Right). YFP signals were quantified and normalized to positive control (cells expressing ABHD5 and ATGL in the absence of perilipin 1). Graphs are presented as mean ± SD of three independent experiments. ***P < 0.001; ns, not significant.