Fig. 3.

EM of PIV5 F-GCNt samples subjected to 21 °C, 45 °C, 55 °C, or 65 °C temperatures for 20 s. (A–E) Prefusion F-GCNt protein was exposed to heating in the presence of H₂O₂ for 20 s, as in the FPOP labeling experiment, but without laser exposure. The prefusion and postfusion samples were both exposed to 21 °C (room temperature). (F) Approximately 300 trimers were counted from four to eight fields of view for each sample condition and plotted as the percentage of total trimers that were postfusion trimers. Error bars represent ±1 SD. P values were calculated using a two-tailed Student t test (**P < 0.01; ***P < 0.001; n.s., not significant).