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. 1999 Jan 15;103(2):229–238. doi: 10.1172/JCI5487

Figure 1.

Figure 1

Targeting strategy, Southern blots and PCR analysis of ES cells and mice lacking the β3-integrin gene. (a) Structure of β3 targeting construct, wild-type β3 allele and targeted β3 allele. Exons are indicated as black boxes. The 1.7 kb pgk-neomycin-resistance cassette replaces a 1.4 kb HindIII fragment of the β3-gene including exons I and II. Pgk-thymidine kinase genes were used for negative selection. (b) Southern blot analysis of EcoRI, BstEII and NcoI-digested genomic DNA from a representative targeted clone (111). Probe A lay 5′ of the targeting construct and probe B lay inside it (a). (c) PCR analysis of wild-type, heterozygous and β3-null tail DNA used to screen offspring. Primer locations are indicated by half arrows in A and give products of 446 bp with primers 1 and 3 (wild-type) or 538 bp with primers 1 and 2 (mutant).